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protease inhibitor  (Valiant Co Ltd)

 
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    Valiant Co Ltd protease inhibitor
    Protease Inhibitor, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 560 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor/product/Valiant Co Ltd
    Average 96 stars, based on 560 article reviews
    protease inhibitor - by Bioz Stars, 2026-05
    96/100 stars

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    Cellular uptake and lysosomal escape of CAMs. (a), (b) Fluorescence images and normalized fluorescence quantification of chondrocytes and meniscus cells after the uptake of different materials, as well as the cellular uptake efficiency results. red: fluorescently labeled materials; green: cell membrane; scale bar: 10 μm; n = 3; one-way ANOVA; ∗∗ represents p < 0.01; ∗∗∗ represents p < 0.0005; and ns represents no significant difference. (c, d) Flow cytometry analysis and normalized fluorescence quantification of chondrocytes (c) and meniscus cells (d) taking up CAMs after exposure to different <t>inhibitors.</t> n = 3; one-way ANOVA; ∗ represents p < 0.05; ∗∗ represents p < 0.01; ∗∗∗ represents p < 0.0005; ∗∗∗∗ represents p < 0.0001; and ns represents no significant difference. (e) Lysosomal escape of chondrocytes incubated with PF or PFMPCr/DS CAMs for 5 min, 1 h, or 6 h. Blue: nuclei; red: fluorescently labeled materials; green: lysosomes; scale bar: 10 μm. (f) Lysosomal escape of meniscus cells incubated with PF or PFMPCr/DS CAMs for 5 min, 1 h, or 6 h. Blue: nuclei; red: fluorescently labeled materials; green: lysosomes; scale bar: 10 μm.
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    Cellular uptake and lysosomal escape of CAMs. (a), (b) Fluorescence images and normalized fluorescence quantification of chondrocytes and meniscus cells after the uptake of different materials, as well as the cellular uptake efficiency results. red: fluorescently labeled materials; green: cell membrane; scale bar: 10 μm; n = 3; one-way ANOVA; ∗∗ represents p < 0.01; ∗∗∗ represents p < 0.0005; and ns represents no significant difference. (c, d) Flow cytometry analysis and normalized fluorescence quantification of chondrocytes (c) and meniscus cells (d) taking up CAMs after exposure to different <t>inhibitors.</t> n = 3; one-way ANOVA; ∗ represents p < 0.05; ∗∗ represents p < 0.01; ∗∗∗ represents p < 0.0005; ∗∗∗∗ represents p < 0.0001; and ns represents no significant difference. (e) Lysosomal escape of chondrocytes incubated with PF or PFMPCr/DS CAMs for 5 min, 1 h, or 6 h. Blue: nuclei; red: fluorescently labeled materials; green: lysosomes; scale bar: 10 μm. (f) Lysosomal escape of meniscus cells incubated with PF or PFMPCr/DS CAMs for 5 min, 1 h, or 6 h. Blue: nuclei; red: fluorescently labeled materials; green: lysosomes; scale bar: 10 μm.
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    Image Search Results


    Cellular uptake and lysosomal escape of CAMs. (a), (b) Fluorescence images and normalized fluorescence quantification of chondrocytes and meniscus cells after the uptake of different materials, as well as the cellular uptake efficiency results. red: fluorescently labeled materials; green: cell membrane; scale bar: 10 μm; n = 3; one-way ANOVA; ∗∗ represents p < 0.01; ∗∗∗ represents p < 0.0005; and ns represents no significant difference. (c, d) Flow cytometry analysis and normalized fluorescence quantification of chondrocytes (c) and meniscus cells (d) taking up CAMs after exposure to different inhibitors. n = 3; one-way ANOVA; ∗ represents p < 0.05; ∗∗ represents p < 0.01; ∗∗∗ represents p < 0.0005; ∗∗∗∗ represents p < 0.0001; and ns represents no significant difference. (e) Lysosomal escape of chondrocytes incubated with PF or PFMPCr/DS CAMs for 5 min, 1 h, or 6 h. Blue: nuclei; red: fluorescently labeled materials; green: lysosomes; scale bar: 10 μm. (f) Lysosomal escape of meniscus cells incubated with PF or PFMPCr/DS CAMs for 5 min, 1 h, or 6 h. Blue: nuclei; red: fluorescently labeled materials; green: lysosomes; scale bar: 10 μm.

    Journal: Bioactive Materials

    Article Title: Artificial mitochondria ameliorates osteoarthritis through restoring cellular energy metabolism homeostasis

    doi: 10.1016/j.bioactmat.2026.02.028

    Figure Lengend Snippet: Cellular uptake and lysosomal escape of CAMs. (a), (b) Fluorescence images and normalized fluorescence quantification of chondrocytes and meniscus cells after the uptake of different materials, as well as the cellular uptake efficiency results. red: fluorescently labeled materials; green: cell membrane; scale bar: 10 μm; n = 3; one-way ANOVA; ∗∗ represents p < 0.01; ∗∗∗ represents p < 0.0005; and ns represents no significant difference. (c, d) Flow cytometry analysis and normalized fluorescence quantification of chondrocytes (c) and meniscus cells (d) taking up CAMs after exposure to different inhibitors. n = 3; one-way ANOVA; ∗ represents p < 0.05; ∗∗ represents p < 0.01; ∗∗∗ represents p < 0.0005; ∗∗∗∗ represents p < 0.0001; and ns represents no significant difference. (e) Lysosomal escape of chondrocytes incubated with PF or PFMPCr/DS CAMs for 5 min, 1 h, or 6 h. Blue: nuclei; red: fluorescently labeled materials; green: lysosomes; scale bar: 10 μm. (f) Lysosomal escape of meniscus cells incubated with PF or PFMPCr/DS CAMs for 5 min, 1 h, or 6 h. Blue: nuclei; red: fluorescently labeled materials; green: lysosomes; scale bar: 10 μm.

    Article Snippet: Total protein was extracted using RIPA lysis buffer (C1053, Applygen, China) containing protease inhibitors (P1265-1, Applygen, China) and phosphatase inhibitors (P1260-1, Applygen, China).

    Techniques: Fluorescence, Labeling, Membrane, Flow Cytometry, Incubation